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recombinant rat gdnf  (R&D Systems)


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    Structured Review

    R&D Systems recombinant rat gdnf
    Recombinant Rat Gdnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+rat+gdnf/Recombinant+Rat+CNTF+Protein/pm41972906-64-17-23
    Average 93 stars, based on 42 article reviews
    recombinant rat gdnf - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: TBK1 activity regulates the directionality of axonal transport of signalling endosomes
    Article Snippet: Cells were routinely tested for Mycoplasma using a MycoAlert detection kit (Lonza). .. MNs were resuspended in motor neuron medium (Neurobasal, 2% heat-inactivated horse serum, 2% B27 supplement, 1X GlutaMAX, 25 μM 2-mercaptoethanol, 1% penicillin/streptomycin, recombinant rat CNTF [10 ng/ml; R&D Systems], recombinant rat GDNF [0.1 ng/ml; R&D Systems], and recombinant human BDNF [1 ng/ml; R&D Systems]). .. MNs were resuspended in motor neuron medium (Neurobasal, 2% heat-inactivated horse serum, 2% B27 supplement, 1X GlutaMAX, 25 μM 2-mercaptoethanol, 1% penicillin/streptomycin, recombinant rat CNTF [10 ng/ml; R&D Systems], recombinant rat GDNF [0.1 ng/ml; R&D Systems], and recombinant human BDNF [1 ng/ml; R&D Systems]).

    Article Title: Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice
    Article Snippet: .. Mouse spermatogonial stem cell (mSSC) lines were derived from the P5.5 testes with the genetic background of B6D2F1 (C57BL/6 × DBA). mSSCs were cultured as previously described: StemPro‐34 SFM (Invitrogen) supplemented with StemPro supplement (Invitrogen), 1% FBS, N2 (100×), 6 mg/mL D‐(1)‐glucose, 5 mg/mL BSA, 0.1 mM NEAA, 1 mM sodium pyruvate, 0.1 mM 2‐mercaptoethanol, 2 mM L‐glutamine, 10 −4 M ascorbic acid, 10 mg/mL biotin, 30 ng/mL β‐estradiol, 60 ng/mL progesterone (Sigma), 20 ng/mL mouse EGF, 10 ng/mL human bFGF, 10 ng/mL recombinant rat GDNF (R&D Systems) and 10 3 U/mL LIF (Merck Millipore). ..

    Article Title: TBK1 activity regulates the directionality of axonal transport of signalling endosomes
    Article Snippet: .. MNs were resuspended in motor neuron medium (Neurobasal, 2% heat-inactivated horse serum, 2% B27 supplement, 1X GlutaMax, 25 μM 2-mercaptoethanol, 1% penicillin/streptomycin, recombinant rat CNTF (R&D Systems, 10 ng/ml), recombinant rat GDNF (R&D Systems, 0.1 ng/ml) and recombinant human BDNF (R&D Systems, 1 ng/ml)). .. Magnetofection (NeuroMag, OZ Biosciences) was carried out following manufacturer’s instructions (with 0.5 μg of DNA, 1.75 μl beads and 15 min of incubation on top of a permanent magnet).

    Article Title:
    Article Snippet: .. SSC medium: SSC base supplemented with StemPro Supplement (Invitrogen 10639), 20 ng/mL mouse epidermal growth factor (EGF; Becton Dickinson 40010), 10 ng/mL human basic fibroblast growth factor (bFGF; Becton Dickinson 13256-029), 1000 U/mL ESGRO (murine leukemia inhibitory factor, Chemicon ESG1107), 10 ng/mL recombinant rat GDNF (R&D Systems 512-GF). .. The growth factors (EGF, bFGF, ESGRO [LIF], GDNF) and StemPro Supplement should also be prepared as aliquots and stored at −20°C.

    Article Title: Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice.
    Article Snippet: .. Mouse spermatogonial stem cell (mSSC) lines were derived from the P5.5 testes with the genetic background of B6D2F1 (C57BL/6 DBA). mSSCs were cultured as previously described: StemPro-34 SFM (Invitrogen) supplemented with StemPro supplement (Invitrogen), 1% FBS, N2 (100 ), 6 mg/mL D-(1)-glucose, 5 mg/mL BSA, 0.1 mM NEAA, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 2 mM L-glutamine, 10 4 M ascorbic acid, 10 mg/mL biotin, 30 ng/mL β-estradiol, 60 ng/mL progesterone (Sigma), 20 ng/mL mouse EGF, 10 ng/mL human bFGF, 10 ng/mL recombinant rat GDNF (R&D Systems) and 103 U/mL LIF (Merck Millipore). ..

    Article Title: GDNF facilitates cognitive function recovery following neonatal surgical-induced learning and memory impairment via activation of the RET pathway and modulation of downstream effectors PKMζ and Kalirin in rats.
    Article Snippet: .. Intracerebroventricular injection of GDNF Rats from GDNF group, Surg+GDNF group and Surg+GDNF+BBT group received right intracerebroventricular injection of 0.3 μg recombinant rat GDNF (catalogue number: 512-GF; R&D Systems, Minneapolis, MN) in 3 μl PBS, as outlined in relevant literature [18,22]. ..

    Article Title: Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells.
    Article Snippet: Skeletal muscle has the capacity to regenerate after injury or disease,1 and this regenerative capacity is due to populations of myogenic precursor cells found within the muscles.2,3 More recently, it has been shown that normal adult skeletal muscles have a slow rate of myonuclear turnover throughout life, occurring at apparently random locations along the length of individual myofibers.4,5 This process is even more striking in the extraocular muscles, which show a high rate of myonuclear turnover throughout life in the absence of injury or skeletal muscle-related diseases.4,6,7 Based on our studies and those of other laboratories, this may be due, in part, to the presence of two distinct populations of myogenic precursor cells within the extraocular muscles.. In addition to the PAX7positive satellite cells found in all skeletal muscles,8 we 1435666 JHCXXX10.1369/00221554261435666CNTF, GDNF and EOM Precursor CellsWinker et al. research-article2026

    Derivative Assay:

    Article Title: Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice
    Article Snippet: .. Mouse spermatogonial stem cell (mSSC) lines were derived from the P5.5 testes with the genetic background of B6D2F1 (C57BL/6 × DBA). mSSCs were cultured as previously described: StemPro‐34 SFM (Invitrogen) supplemented with StemPro supplement (Invitrogen), 1% FBS, N2 (100×), 6 mg/mL D‐(1)‐glucose, 5 mg/mL BSA, 0.1 mM NEAA, 1 mM sodium pyruvate, 0.1 mM 2‐mercaptoethanol, 2 mM L‐glutamine, 10 −4 M ascorbic acid, 10 mg/mL biotin, 30 ng/mL β‐estradiol, 60 ng/mL progesterone (Sigma), 20 ng/mL mouse EGF, 10 ng/mL human bFGF, 10 ng/mL recombinant rat GDNF (R&D Systems) and 10 3 U/mL LIF (Merck Millipore). ..

    Article Title: Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice.
    Article Snippet: .. Mouse spermatogonial stem cell (mSSC) lines were derived from the P5.5 testes with the genetic background of B6D2F1 (C57BL/6 DBA). mSSCs were cultured as previously described: StemPro-34 SFM (Invitrogen) supplemented with StemPro supplement (Invitrogen), 1% FBS, N2 (100 ), 6 mg/mL D-(1)-glucose, 5 mg/mL BSA, 0.1 mM NEAA, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 2 mM L-glutamine, 10 4 M ascorbic acid, 10 mg/mL biotin, 30 ng/mL β-estradiol, 60 ng/mL progesterone (Sigma), 20 ng/mL mouse EGF, 10 ng/mL human bFGF, 10 ng/mL recombinant rat GDNF (R&D Systems) and 103 U/mL LIF (Merck Millipore). ..

    Cell Culture:

    Article Title: Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice
    Article Snippet: .. Mouse spermatogonial stem cell (mSSC) lines were derived from the P5.5 testes with the genetic background of B6D2F1 (C57BL/6 × DBA). mSSCs were cultured as previously described: StemPro‐34 SFM (Invitrogen) supplemented with StemPro supplement (Invitrogen), 1% FBS, N2 (100×), 6 mg/mL D‐(1)‐glucose, 5 mg/mL BSA, 0.1 mM NEAA, 1 mM sodium pyruvate, 0.1 mM 2‐mercaptoethanol, 2 mM L‐glutamine, 10 −4 M ascorbic acid, 10 mg/mL biotin, 30 ng/mL β‐estradiol, 60 ng/mL progesterone (Sigma), 20 ng/mL mouse EGF, 10 ng/mL human bFGF, 10 ng/mL recombinant rat GDNF (R&D Systems) and 10 3 U/mL LIF (Merck Millipore). ..

    Article Title: Dynamic transcriptomic and regulatory networks underpinning the transition from fetal primordial germ cells to spermatogonia in mice.
    Article Snippet: .. Mouse spermatogonial stem cell (mSSC) lines were derived from the P5.5 testes with the genetic background of B6D2F1 (C57BL/6 DBA). mSSCs were cultured as previously described: StemPro-34 SFM (Invitrogen) supplemented with StemPro supplement (Invitrogen), 1% FBS, N2 (100 ), 6 mg/mL D-(1)-glucose, 5 mg/mL BSA, 0.1 mM NEAA, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 2 mM L-glutamine, 10 4 M ascorbic acid, 10 mg/mL biotin, 30 ng/mL β-estradiol, 60 ng/mL progesterone (Sigma), 20 ng/mL mouse EGF, 10 ng/mL human bFGF, 10 ng/mL recombinant rat GDNF (R&D Systems) and 103 U/mL LIF (Merck Millipore). ..

    Injection:

    Article Title: GDNF facilitates cognitive function recovery following neonatal surgical-induced learning and memory impairment via activation of the RET pathway and modulation of downstream effectors PKMζ and Kalirin in rats.
    Article Snippet: .. Intracerebroventricular injection of GDNF Rats from GDNF group, Surg+GDNF group and Surg+GDNF+BBT group received right intracerebroventricular injection of 0.3 μg recombinant rat GDNF (catalogue number: 512-GF; R&D Systems, Minneapolis, MN) in 3 μl PBS, as outlined in relevant literature [18,22]. ..

    other:

    Article Title: TBK1 activity regulates the directionality of axonal transport of signalling endosomes.
    Article Snippet: The in vitro kinase validation was carried out by ProQinase GmbH, using a radiometric protein kinase filter–binding assay and a library of 190 serine/threonine kinases, using His6-Rab7 WT or S72P as substrates.



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